The ancestral duplicated <i>DL/CRC</i> orthologs, <i>PeDL1</i> and <i>PeDL2</i>, function in orchid reproductive organ innovation
نویسندگان
چکیده
Abstract Orchid gynostemium, the fused organ of androecium and gynoecium, ovule development are unique developmental processes. Two DROOPING LEAF/CRABS CLAW (DL/CRC) genes, PeDL1 PeDL2, were identified from Phalaenopsis orchid genome functionally characterized. Phylogenetic analysis indicated that most recent common ancestor orchids contained duplicated DL/CRC-like genes. Temporal spatial expression PeDL genes specifically expressed in gynostemium at early stages development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Virus-induced gene silencing (VIGS) PeDL2 affected number protuberant initials differentiated placenta. Transient overexpression caused abnormal stigmatic cavity gynostemium. PeDL1, but not form a heterodimer with equestris CINCINNATA 8 (PeCIN8). Paralogous retention subsequent divergence sequences P. might result differentiation function protein behaviors. These results reveal ancestral play important roles reproductive innovation.
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2021
ISSN: ['0022-0957', '1460-2431']
DOI: https://doi.org/10.1093/jxb/erab195